LRP1 antibody

Catalog No. ABIN337070

Product Details anti-LRP1 Antibody

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LRP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes human CD91, a type I membrane protein which is also known as the alpha 2 macroglobulin receptor (alpha 2 MR). Recognizes an epitope in the extracellular region of the 500kD alpha chain.

Purification: Protein G purified

Immunogen: Purified alpha 2 macroglobulin receptor.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P (5 - 10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5-10 μg/mL. Flow Cytometry: 1:50-1:100. Use 10 μL to label 10^6 cells in 100 μL. Western Blot: non-reducing conditions. Positive control: Liver.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for LRP1

Target: LRP1 (Low Density Lipoprotein Receptor-Related Protein 1 (LRP1))

Alternative Name: LRP1 / CD91 (LRP1 Products)

Background: Name/Gene ID: LRP1

Synonyms: LRP1, Apolipoprotein E receptor, CD91 antigen, CD91, A2MR, IGFBP3R, Alpha-2-macroglobulin receptor, TGFBR5, Type V tgf-beta receptor, APOER, APR, LRP, LRP-1

Gene ID: 4035

UniProt: Q07954

Pathways: Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling