Occludin antibody (C-Term)

Catalog No. ABIN337048

Product Details anti-Occludin Antibody

Target: Occludin (OCLN)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.

Purification: Protein A purified

Immunogen: GST fusion protein consisting of the C-terminal region (~150aa) of human occludin.

Type of Immunogen: Fusion protein

Clone: OC-3F10

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 3 μg/mL), IHC, IHC-P (10 μg/mL), WB (0.1 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

References for anti-Occludin antibody (ABIN337048)

Krueger, Härtig, Reichenbach, Bechmann, Michalski: "Blood-brain barrier breakdown after embolic stroke in rats occurs without ultrastructural evidence for disrupting tight junctions." in: PLoS ONE, Vol. 8, Issue 2, pp. e56419, (2013) (PubMed).

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN / Occludin (OCLN Products)

Synonyms: AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody

Background: Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C