HNE antibody

Catalog No. ABIN335414

Product Details anti-HNE Antibody

Target: HNE (4-Hydroxynonenal (HNE))

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: Specific for 4HNE-Lysine, 4-HNE-histidine and 4-HNE cysteine adduct.By inhibition test, this antibody has a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE cysteine adduct. This antibody show almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.

Purification: Ammonium sulphate purified IgG

Immunogen: 4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.

Clone: HNEJ-2

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 1000μL of distilled water.

Buffer: 100ug/mL IgG in 50 mM Tris buffered saline (TBS).

Storage: -20 °C

References for anti-HNE antibody (ABIN335414)

Miyake, Tanabe, Tanimura, Nakashima, Morioka, Masuda, Sugiyama, Sato, Wada: "Genetic Deletion of Vasohibin-2 Exacerbates Ischemia-Reperfusion-Induced Acute Kidney Injury." in: International journal of molecular sciences, Vol. 21, Issue 12, (2020) (PubMed).

Kanbara, Ohkawara, Nakashima, Ohta, Koshimizu, Inoue, Tomita, Ito, Masuda, Ishiguro, Imagama, Ohno: "Zonisamide ameliorates progression of cervical spondylotic myelopathy in a rat model." in: Scientific reports, Vol. 10, Issue 1, pp. 13138, (2020) (PubMed).

Hosohata, Jin, Takai, Iwanaga: "Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis." in: International journal of molecular sciences, Vol. 19, Issue 10, (2019) (PubMed).

Paka, Smith, Jung, McCormack, Zhou, Duan, Li, Shi, Hao, Jiang, Yamin, Goldberg, Narayan: "Anti-steatotic and anti-fibrotic effects of the KCa3.1 channel inhibitor, Senicapoc, in non-alcoholic liver disease." in: World journal of gastroenterology, Vol. 23, Issue 23, pp. 4181-4190, (2018) (PubMed).

Uneda, Wakui, Maeda, Azushima, Kobayashi, Haku, Ohki, Haruhara, Kinguchi, Matsuda, Ohsawa, Minegishi, Ishigami, Toya, Atobe, Yamashita, Umemura, Tamura: "Angiotensin II Type 1 Receptor-Associated Protein Regulates Kidney Aging and Lifespan Independent of Angiotensin." in: Journal of the American Heart Association, Vol. 6, Issue 8, (2018) (PubMed).

Uetake, Ikeda, Irie, Tejima, Matsui, Ogura, Wang, Mu, Hirohama, Ando, Sawamura, Yatomi, Fujita, Shimosawa: "High-salt in addition to high-fat diet may enhance inflammation and fibrosis in liver steatosis induced by oxidative stress and dyslipidemia in mice." in: Lipids in health and disease, Vol. 14, pp. 6, (2016) (PubMed).

Miller, Singh, Wang, Hall: "Nrf2-ARE activator carnosic acid decreases mitochondrial dysfunction, oxidative damage and neuronal cytoskeletal degradation following traumatic brain injury in mice." in: Experimental neurology, Vol. 264, pp. 103-10, (2015) (PubMed).

Apidianakis, Que, Xu, Tegos, Zimniak, Hamblin, Tompkins, Xiao, Rahme: "Down-regulation of glutatione S-transferase α 4 (hGSTA4) in the muscle of thermally injured patients is indicative of susceptibility to bacterial infection." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 730-7, (2012) (PubMed).

Tskitishvili, Sharentuya, Temma-Asano, Mimura, Kinugasa-Taniguchi, Kanagawa, Fukuda, Kimura, Tomimatsu, Shimoya: "Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells." in: Molecular human reproduction, Vol. 16, Issue 3, pp. 188-99, (2010) (PubMed).

Ogawa, Fujii, Yoshizato, Kawada: "A human-type nonalcoholic steatohepatitis model with advanced fibrosis in rabbits." in: The American journal of pathology, Vol. 177, Issue 1, pp. 153-65, (2010) (PubMed).

Wakamatsu, Dogru, Ayako, Takano, Matsumoto, Ibrahim, Okada, Satake, Fukagawa, Shimazaki, Tsubota, Fujishima: "Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease." in: Molecular vision, Vol. 16, pp. 2465-75, (2010) (PubMed).

Raz, Zhang, Zhou, Han, Gulati, Yang, Yang, Wang, Brann: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, (2010) (PubMed).

Kurashige, Abe, Furusu, Miyazaki, Obata, Xia, Nakazawa, Nakazawa, Funakoshi, Harada, Koji, Kohno: "Renoprotective effect of azelnidipine in rats." in: Biological & pharmaceutical bulletin, Vol. 31, Issue 12, pp. 2237-44, (2008) (PubMed).

Amidi, French, Chung, Halsted, Medici, French: "M-30 and 4HNE are sequestered in different aggresomes in the same hepatocytes." in: Experimental and molecular pathology, Vol. 83, Issue 3, pp. 296-300, (2007) (PubMed).

Kodai, Takemura, Minamiyama, Hai, Yamamoto, Kubo, Yoshida, Niki, Okada, Hirohashi, Suehiro: "S-allyl cysteine prevents CCl(4)-induced acute liver injury in rats." in: Free radical research, Vol. 41, Issue 4, pp. 489-97, (2007) (PubMed).

Tanito, Kaidzu, Anderson: "Delayed loss of cone and remaining rod photoreceptor cells due to impairment of choroidal circulation after acute light exposure in rats." in: Investigative ophthalmology & visual science, Vol. 48, Issue 4, pp. 1864-72, (2007) (PubMed).

Kurimoto, Kawano, Tsuji, Hatachi, Jikimoto, Sugiyama, Kasagi, Komori, Nakamura, Yodoi, Kumagai: "Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome." in: The Journal of rheumatology, Vol. 34, Issue 10, pp. 2035-43, (2007) (PubMed).

Hirono, Yoshimoto, Suzuki, Sugiyama, Sakurada, Takai, Kobayashi, Shichiri, Hirata: "Angiotensin II receptor type 1-mediated vascular oxidative stress and proinflammatory gene expression in aldosterone-induced hypertension: the possible role of local renin-angiotensin system." in: Endocrinology, Vol. 148, Issue 4, pp. 1688-96, (2007) (PubMed).

Choi, Ryu, Kim, McLarnon: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, (2007) (PubMed).

Tanito, Elliott, Kotake, Anderson: "Protein modifications by 4-hydroxynonenal and 4-hydroxyhexenal in light-exposed rat retina." in: Investigative ophthalmology & visual science, Vol. 46, Issue 10, pp. 3859-68, (2005) (PubMed).

Target Details for HNE

Target: HNE (4-Hydroxynonenal (HNE))

Abstract: HNE Products

Target Type: Chemical