Smoothelin antibody

Catalog No. ABIN335397

Product Details anti-Smoothelin Antibody

Target: Smoothelin (SMTN)

Reactivity: Human, Pig, Cat, Chicken, Dog, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smoothelin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human, canine, feline, chicken, swine, monkey. Not reactive with rat.

Purification: Purified

Immunogen: R4A is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal extract of chicken gizzard.

Clone: R4A

Isotype: IgG1

Application Details

Application Notes: R4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. R4A is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. For staining paraffin-embedded tissues pretreatment in 10 mM citrate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Concentration: 1mg/ml

Buffer: in PBS containing 0.09% Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Smoothelin antibody (ABIN335397)

Coco, Hirsch, Hornick: "Smoothelin is a specific marker for smooth muscle neoplasms of the gastrointestinal tract." in: The American journal of surgical pathology, Vol. 33, Issue 12, pp. 1795-801, (2009) (PubMed).

Council, Hameed: "Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 5, pp. 639-50, (2009) (PubMed).

Deruiter, Rensen, Coolen, Hierck, Bergwerff, Debie, Gittenberger-De Groot, Van Eys: "Smoothelin expression during chicken embryogenesis: detection of an embryonic isoform." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 4, pp. 460-3, (2001) (PubMed).

Johansson, Eriksson, Ramaekers, Thornell: "Smoothelin in adult and developing human arteries and myocardium." in: Histochemistry and cell biology, Vol. 112, Issue 4, pp. 291-9, (2000) (PubMed).

Johansson, Eriksson, Ramaekers, Thornell: "Smoothelin and intermediate filament proteins in human aortocoronary saphenous vein by-pass grafts." in: The Histochemical journal, Vol. 31, Issue 11, pp. 723-7, (2000) (PubMed).

Christen, Bochaton-Piallat, Neuville, Rensen, Redard, van Eys, Gabbiani: "Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation." in: Circulation research, Vol. 85, Issue 1, pp. 99-107, (1999) (PubMed).

Wehrens, Mies, Gimona, Ramaekers, Van Eys, Small: "Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform." in: FEBS letters, Vol. 405, Issue 3, pp. 315-20, (1997) (PubMed).

van der Loop, Gabbiani, Kohnen, Ramaekers, van Eys: "Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 17, Issue 4, pp. 665-71, (1997) (PubMed).

van der Loop, Schaart, Timmer, Ramaekers, van Eys: "Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 401-11, (1996) (PubMed).

Target Details for Smoothelin

Target: Smoothelin (SMTN)

Alternative Name: Smoothelin (SMTN Products)

Synonyms: smsmo antibody, MGC68765 antibody, SMTN antibody, MGC140000 antibody, MGC155237 antibody, DKFZp468D019 antibody, smoothelin antibody, smoothelin L homeolog antibody, smoothelin b antibody, SMTN antibody, Smtn antibody, smtn.L antibody, smtnb antibody, smtn antibody

Background: Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22.