Lamin B2 antibody

Catalog No. ABIN335392

Product Details anti-Lamin B2 Antibody

Target: Lamin B2 (LMNB2)

Reactivity: Human, Mouse, Xenopus laevis, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lamin B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Specificity: Human, mouse, hamster and Xenopus.

Purification: Purified

Immunogen: LN43 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with the detergent insoluble fraction of potoroo cell line PtK1.

Clone: LN43

Isotype: IgG1

Application Details

Application Notes: LN43 reacts with an epitope located in the C-terminal part of lamin B2. LN43 is suitable for immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Lamin B2 antibody (ABIN335392)

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, (1997) (PubMed).

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, (1997) (PubMed).

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, (1997) (PubMed).

Machiels, Zorenc, Endert, Kuijpers, van Eys, Ramaekers, Broers: "An alternative splicing product of the lamin A/C gene lacks exon 10." in: The Journal of biological chemistry, Vol. 271, Issue 16, pp. 9249-53, (1996) (PubMed).

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, (1996) (PubMed).

Hozák, Sasseville, Raymond, Cook: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, (1995) (PubMed).

Jenkins, Hölman, Lyon, Lane, Stick, Hutchison: "Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix." in: Journal of cell science, Vol. 106 ( Pt 1), pp. 275-85, (1994) (PubMed).

Bridger, Kill, OFarrell, Hutchison: "Internal lamin structures within G1 nuclei of human dermal fibroblasts." in: Journal of cell science, Vol. 104 ( Pt 2), pp. 297-306, (1993) (PubMed).

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Alternative Name: Lamin B2 (LMNB2 Products)

Synonyms: lmnb2-a antibody, LMNB2 antibody, lmn2 antibody, lamb2 antibody, Lamin-L(II) antibody, LOC100136040 antibody, im:7142331 antibody, lamin antibody, wu:fb94e05 antibody, wu:fb95e12 antibody, wu:fc15d06 antibody, wu:fc49h03 antibody, lamin-b2 antibody, lmnb2 antibody, LAMB2 antibody, LMN2 antibody, RGD1563803 antibody, lamin B2 S homeolog antibody, lamin B2 antibody, lamin B2 L homeolog antibody, lmnb2.S antibody, LMNB2 antibody, lmnb2 antibody, lmnb2.L antibody, Lmnb2 antibody

Background: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, ie lamin B1 and lamin B2.

Pathways: Apoptosis, Caspase Cascade in Apoptosis