Selectin E/CD62e antibody

Catalog No. ABIN335384

Product Details anti-Selectin E/CD62e Antibody

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func)

Specificity: Mouse.

Purification: Purified

Immunogen: UZ5 is a rat monoclonal IgG2b antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a Lewis rat immunized with LPS-activated mlEND1 cells expressing E-selectin at their cell surface.

Clone: UZ5

Isotype: IgG2b

Application Details

Application Notes: UZ5 recognizes a conformational epitope in the CR1 region in murine E-selectin. UZ5 partially inhibits leukocyte adhesion to murine activated endothelial cells. UZ5 is suitable for immunoblotting, ELISA, immunoprecipitation, immunohistochemistry on fozen sections and flow cytometry (dilution 1:200).

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Selectin E/CD62e antibody (ABIN335384)

Hammel, Weitz-Schmidt, Krause, Moll, Vestweber, Zerwes, Hallmann: "Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion." in: Experimental cell research, Vol. 269, Issue 2, pp. 266-74, (2001) (PubMed).

Hallmann, Zimmermann, Sorokin, Needham, Von der Mark: "Adhesion of leukocytes to the inflammed endothelium." in: Scandinavian journal of rheumatology. Supplement, Vol. 101, pp. 107-9, (1995) (PubMed).

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: E-selectin (SELE Products)

Synonyms: SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody

Background: Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkappaB dependent signalling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

Pathways: Thromboxane A2 Receptor Signaling