KRT17 antibody

Catalog No. ABIN335340

Product Details anti-KRT17 Antibody

Target: KRT17 (Keratin 17 (KRT17))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Human and rat.

Purification: Purified

Immunogen: E3 is a mouse monoclonal IgG2b antibody derived by fusion of X63 Ag 8.653 mouse myeloma cells with spleen cells from a Balb/c mouse immunized with a cytoskeletal preparation from rat colon.

Clone: E3

Isotype: IgG2b

Application Details

Application Notes: E3 reacts with cytokeratin 17 in basal layers of pseudo-stratified and transitional epithelia. APPLICATIONS E3 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-KRT17 antibody (ABIN335340)

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, (1996) (PubMed).

Smedts, Ramaekers, Link, Lauerova, Troyanovsky, Schijf, Vooijs: "Detection of keratin subtypes in routinely processed cervical tissue: implications for tumour classification and the study of cervix cancer aetiology." in: Virchows Archiv : an international journal of pathology, Vol. 425, Issue 2, pp. 145-55, (1994) (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, (1992) (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Robben, Lane, Leigh, Plantema, Vooijs: "Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 140, Issue 3, pp. 601-12, (1992) (PubMed).

Wetzels, Schaafsma, Leigh, Lane, Troyanovsky, Wagenaar, Vooijs, Ramaekers: "Laminin and type VII collagen distribution in different types of human lung carcinoma: correlation with expression of keratins 14, 16, 17 and 18." in: Histopathology, Vol. 20, Issue 4, pp. 295-303, (1992) (PubMed).

de Jong, van Vlijmen, van Erp, Ramaekers, Troyanovski, van de Kerkhof: "Keratin 17: a useful marker in anti-psoriatic therapies." in: Archives of dermatological research, Vol. 283, Issue 7, pp. 480-2, (1992) (PubMed).

Wetzels, Kuijpers, Lane, Leigh, Troyanovsky, Holland, van Haelst, Ramaekers: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, (1991) (PubMed).

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, (1991) (PubMed).

Troyanovsky, Guelstein, Tchipysheva, Krutovskikh, Bannikov: "Patterns of expression of keratin 17 in human epithelia: dependency on cell position." in: Journal of cell science, Vol. 93 ( Pt 3), pp. 419-26, (1990) (PubMed).

Guelstein, Tchypysheva, Ermilova, Litvinova, Troyanovsky, Bannikov: "Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 42, Issue 2, pp. 147-53, (1988) (PubMed).

Target Details for KRT17

Target: KRT17 (Keratin 17 (KRT17))

Alternative Name: Cytokeratin 17 / Keratin K17 (KRT17 Products)

Synonyms: K17 antibody, Krt1-17 antibody, PC antibody, PC2 antibody, PCHC1 antibody, Ka17 antibody, KRT17 antibody, krt16 antibody, si:dkeyp-113d7.7 antibody, KRT14 antibody, keratin 17 antibody, keratin 17 L homeolog antibody, keratin 17, type I antibody, keratin, type I cytoskeletal 17 antibody, Krt17 antibody, KRT17 antibody, krt17 antibody, krt17.L antibody, LOC100737113 antibody, LOC102177275 antibody

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.