Cytokeratin 7 antibody

Catalog No. ABIN335336

Product Details anti-Cytokeratin 7 Antibody

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Human exclusively.

Purification: Purified

Immunogen: OVTL12/30 is a mouse monoclonal IgG1/kappa antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.

Clone: OVTL12-30

Isotype: IgG1

Application Details

Application Notes: OVTL12/30 reacts exlcusively with cytokeratin 7 which is present in specific glandular-type epithelia and most carcinomas derived thereof. OVTL12/30 is effective on frozen tissue after fixation in methanol. It is particularly effective on paraffin-embedded tissue, for which pretreatment with e.g. 0.1% pronase / 0.1% trypsine in PBS or 0.5% pepsine in 0.01N HCl or microwave pretreatment is required. In addition, OVTL 12/30 is suitable for immunocytochemistry, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Cytokeratin 7 antibody (ABIN335336)

Torenbeek, Lagendijk, Van Diest, Bril, van de Molengraft, Meijer: "Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma." in: Histopathology, Vol. 32, Issue 1, pp. 20-7, (1998) (PubMed).

van Niekerk, Vooijs, Casparie-van Velsen, Poels: "Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies." in: Cancer detection and prevention, Vol. 21, Issue 3, pp. 247-57, (1997) (PubMed).

van de Molengraft, van Niekerk, Jap, Poels: "OV-TL 12/30 (keratin 7 antibody) is a marker of glandular differentiation in lung cancer." in: Histopathology, Vol. 22, Issue 1, pp. 35-8, (1993) (PubMed).

van Niekerk, Jap, Ramaekers, van de Molengraft, Poels: "Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues." in: The Journal of pathology, Vol. 165, Issue 2, pp. 145-52, (1992) (PubMed).

van Niekerk, Boerman, Ramaekers, Poels: "Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas." in: The American journal of pathology, Vol. 138, Issue 2, pp. 455-63, (1991) (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin 7 / Keratin K7 (KRT7 Products)

Synonyms: CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.