Keratin 10 antibody

Catalog No. ABIN335328

Product Details anti-Keratin 10 Antibody

Target: Keratin 10 (KRT10)

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Human, mouse, rat, canine.

Purification: Purified

Immunogen: RKSE60 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with cytokeratins from the human epidermis.

Clone: RKSE60

Isotype: IgG1

Application Details

Application Notes: RKSE60 reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. RKSE60 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Keratin 10 antibody (ABIN335328)

Meek, Van Elssen, Huijskens, van der Stegen, Tonnaer, Lumeij, Vanderlocht, Hesselink, Kirkland, Germeraad, Bos: "T cells fail to develop in the human skin-cell explants system, an inconvenient truth." in: BMC immunology, Vol. 12, Issue 1, pp. 17, (2011) (PubMed).

Franssen, Boezeman, Van De Kerkhof, Van Erp: "Monitoring hyperproliferative disorders in human skin: flow cytometry of changing cytokeratin expression." in: Cytometry. Part B, Clinical cytometry, Vol. 57, Issue 1, pp. 32-9, (2003) (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

Schaafsma, Ramaekers, van Muijen, Lane, Leigh, Robben, Huijsmans, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, (1990) (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, (1990) (PubMed).

van Erp, Rijzewijk, Boezeman, Leenders, de Mare, Schalkwijk, van de Kerkhof, Ramaekers, Bauer: "Flow cytometric analysis of epidermal subpopulations from normal and psoriatic skin using monoclonal antibodies against intermediate filaments." in: The American journal of pathology, Vol. 135, Issue 5, pp. 865-70, (1989) (PubMed).

Broers, Carney, Klein Rot, Schaart, Lane, Vooijs, Ramaekers: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, (1987) (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, (1987) (PubMed).

Bijman, Wagener, van Rennes, Wessels, Ramaekers, van den Broek: "Modulation of placental alkaline phosphatase activity and cytokeratins in human HN-1 cells by butyrate, retinoic acid, catecholamines and histamine." in: British journal of cancer, Vol. 56, Issue 2, pp. 127-32, (1987) (PubMed).

Puts, Moesker, Kenemans, Vooijs, Ramaekers: "Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 4, Issue 4, pp. 300-13, (1986) (PubMed).

Ramaekers, Puts, Moesker, Kant, Huysmans, Haag, Jap, Herman, Vooijs: "Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview." in: The Histochemical journal, Vol. 15, Issue 7, pp. 691-713, (1983) (PubMed).

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Cytokeratin 10 / Keratin K10 (KRT10 Products)

Synonyms: BCIE antibody, BIE antibody, CK10 antibody, EHK antibody, K10 antibody, KPP antibody, D130054E02Rik antibody, K1C1 antibody, Krt-1.10 antibody, Krt1-10 antibody, Ka10 antibody, Ker10 antibody, KRT10 antibody, KRT25 antibody, KRT27 antibody, KRT28 antibody, keratin 10 antibody, keratin 10, type I S homeolog antibody, keratin, type I cytoskeletal 25 antibody, KRT10 antibody, Krt10 antibody, krt10.S antibody, LOC101085587 antibody

Background: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.