Collagen IV antibody

Catalog No. ABIN335193

Product Details anti-Collagen IV Antibody

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivity: Human, Rat, Pig, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: Human, rat, pig, cow.

Purification: Antiserum

Immunogen: This polyclonal type IV collagen antibody is a rabbit polyclonal antibody. The rabbits were immunized with isolated human type IV collagen. The immunogen has been isolated from human placenta.

Application Details

Application Notes: Polyclonal type IV collagen recognizes the extracellular - basement membranes. Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2. For immunohistochemistry on paraffin sections the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room temperature. Optimal antibody dilution should be determined by titration, 1:10 - 1:20 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

References for anti-Collagen IV antibody (ABIN335193)

Cleutjens, Havenith, Kasper, Vallinga, Bosman: "Absence of type IV collagen in the centre of the corneal epithelial basement membrane." in: The Histochemical journal, Vol. 22, Issue 12, pp. 688-94, (1991) (PubMed).

Schapers, Pauwels, Havenith, Smeets, van den Brandt, Bosman: "Prognostic significance of type IV collagen and laminin immunoreactivity in urothelial carcinomas of the bladder." in: Cancer, Vol. 66, Issue 12, pp. 2583-8, (1991) (PubMed).

Cleutjens, Havenith, Beek, Vallinga, Ten Kate, Bosman: "Origin of basement membrane type IV collagen in xenografted human epithelial tumor cell lines." in: The American journal of pathology, Vol. 136, Issue 5, pp. 1165-72, (1990) (PubMed).

Visser, van der Beek, Havenith, Cleutjens, Bosman: "Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia." in: Histopathology, Vol. 10, Issue 2, pp. 171-80, (1986) (PubMed).

Target Details for Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Alternative Name: Collagen Type IV (COL4 Products)

Synonyms: collagen, type IV antibody, Let-2 antibody