S100A9 antibody (Internal Region)

Catalog No. ABIN335161

Product Details anti-S100A9 Antibody

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: S100A9

Sequence: DTNADKQLSF EEF

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-DTNADKQLSFEEF

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Lung. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 13-14 kDa band observed in Human Bone Marrow lysates and approx 14-15 kDa in Human Gastrointestinal cancer lysates (calculated MW of 13.2 kDa according to NP_002956.1). Recommended concentration: 0.5-2 μg/mL. Primary incubation 1 hour a

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9 (S100A9 Products)

Background: S100A9, S100 calcium binding protein A9, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B), calgranulin B

Gene ID: 6280

NCBI Accession: NP_002956

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins