Enkephalin antibody (Internal Region)

Catalog No. ABIN335158

Product Details anti-Enkephalin Antibody

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Binding Specificity: Internal Region

Reactivity: Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Enkephalin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: Proenkephalin (mouse)

Sequence: YKDSSKQDES H

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-YKDSSKQDESH, from the internal region of the protein sequence according to NP_001002927.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended

Western Blot: Preliminary experiments gave an approx 40 kDa band in Rat Brain lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8 kDa

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Enkephalin

Target: Enkephalin (PENK) (Proenkephalin (PENK))

Alternative Name: PENK (PENK Products)

Synonyms: AI326464 antibody, ENK antibody, PPA antibody, Penk1 antibody, PENK antibody, penk antibody, Enk antibody, Penk-rs antibody, Penk2 antibody, penkl antibody, zgc:73353 antibody, Penk antibody, proenkephalin antibody, preproenkephalin antibody, proenkephalin b antibody, proenkephalin L homeolog antibody, proenkephalin a antibody, PENK antibody, Penk antibody, penk antibody, penkb antibody, penk.L antibody, penka antibody

Background: Proenkephalin

Gene ID: 18619, 29237

NCBI Accession: NP_001002927

Pathways: Stem Cell Maintenance