FBXO43 antibody (C-Term)

Catalog No. ABIN335134

Product Details anti-FBXO43 Antibody

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXO43 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: EMI2

Sequence: PGSAQSKRNL KRL

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-PGSAQSKRNLKRL, from the C Terminus of the protein sequence according to NP_001025031.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in Mouse Ovary lysates (calculated MW of 78.4 kDa according to NP_001025031.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human, Rat and Pig Testi

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FBXO43 antibody (ABIN335134)

Bernhardt, Kong, Kim, OHalloran, Woodruff: "A zinc-dependent mechanism regulates meiotic progression in mammalian oocytes." in: Biology of reproduction, Vol. 86, Issue 4, pp. 114, (2012) (PubMed).

Perrard, Chassaing, Montillet, Sabido, Durand: "Cytostatic factor proteins are present in male meiotic cells and beta-nerve growth factor increases mos levels in rat late spermatocytes." in: PLoS ONE, Vol. 4, Issue 10, pp. e7237, (2009) (PubMed).

Target Details for FBXO43

Target: FBXO43 (F-Box Protein 43 (FBXO43))

Alternative Name: FBXO43 (FBXO43 Products)

Synonyms: EMI2 antibody, ERP1 antibody, FBX43 antibody, 4930533G20Rik antibody, Emi2 antibody, emi2 antibody, erp1 antibody, Xerp1 antibody, zgc:66488 antibody, F-box protein 43 antibody, F-box protein 43 L homeolog antibody, FBXO43 antibody, Fbxo43 antibody, fbxo43 antibody, fbxo43.L antibody

Background: FBXO43, EMI2, F-box protein 43, Fbx43, early mitotic inhibitor 2, ERP1

Gene ID: 286151, 78803, 315034

NCBI Accession: NP_001025031