ARNTL antibody (Internal Region)

Catalog No. ABIN335124

Product Details anti-ARNTL Antibody

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ARNTL antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: BMAL1 / ARNTL

Sequence: REKITTNCYK FKIKD

Specificity: This antibody is expected to recognize both reported isoforms (NP_001025444.1, NP_001025443.1). Reported variants represent identical protein (NP_001025443.1, NP_001169.3).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence REKITTNCYKFKIKD, from the internal region of the protein sequence according to NP_001169.3, NP_001025444.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx. 70+75 kDa bands observed in Human Brain (Cerebellum) lysates (calculated MW of 68.7 kDa according to NP_001169.3 and 64.1 kDa according to NP_001025444.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ARNTL

Target: ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))

Alternative Name: ARNTL (ARNTL Products)

Background: ARNTL, BMAL1, aryl hydrocarbon receptor nuclear translocator-like, BMAL1c, JAP3, MGC47515, MOP3, PASD3, TIC, ARNT-like protein 1, brain and muscle, bHLH-PAS protein JAP3, basic-helix-loop-helix-PAS orphan MOP3, member of PAS superfamily 3

Gene ID: 406, 11865, 29657

NCBI Accession: NP_001169, NP_001025444

Pathways: Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, Warburg Effect