ZEB1 antibody (Internal Region)

Catalog No. ABIN335118

Product Details anti-ZEB1 Antibody

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: AREB6 / ZEB1

Sequence: RPQIRQKIEN KPLQE

Specificity: This antibody is expected to recognize both reported isoforms (NP_001121600.1, NP_110378.3).

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RPQIRQKIENKPLQE, from the internal region of the protein sequence according to NP_001121600.1, NP_110378.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx.100 kDa band observed in Mouse Heart lysates (calculated MW of 122 kDa according to Human NP_110378.3 and Mouse NP_035676.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1 (ZEB1 Products)

Background: ZEB1, AREB6, zinc finger E-box binding homeobox 1, BZP, DELTA-EF1, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, OTTHUMP00000019405, transcription factor 8 (represses interleukin 2 expression), zinc finger homeodomain enhancer-binding prote

Gene ID: 6935, 21417, 25705

NCBI Accession: NP_001121600, NP_110378

Pathways: Regulation of Muscle Cell Differentiation