Ascorbate Oxidase antibody

Catalog No. ABIN334592

Product Details anti-Ascorbate Oxidase Antibody

Target: Ascorbate Oxidase (AO)

Reactivity: Pumpkin

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ascorbate Oxidase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 61 / 45 kDa(Cucurbita pepo)

Purification: affinity purified

Immunogen: ascorbate oxidase purified from Cucurbita sp.

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 1000 (WB), 1 : 1000 - 1 : 30 000 (ELISA).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 0.5 mL of sterile water

Buffer: PBS pH 7.2

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Working dilutions should be stored at 4 °C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20 °C. Working dilutions should be stored at 4 °C, not refrozen and prefarably used the same day.

Target Details for Ascorbate Oxidase

Target: Ascorbate Oxidase (AO)

Abstract: AO Products

Synonyms: AO antibody, ascorbate oxidase antibody, L-ascorbate oxidase antibody, AO antibody, LOC9310099 antibody

Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90 % of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Molecular Weight: expected: 61 kDa, apparent: 45 kDa (Cucurbita pepo)