Amylase beta antibody (Biotin)

Catalog No. ABIN334590

Product Details anti-Amylase beta Antibody (Biotin)

Target: Amylase beta (amyB)

Reactivity: Potato

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Amylase beta antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Not reactive in: n.a.

Predicted Reactivity: dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis, Vitis vinifera, trees: Populus trichocarpa, Physcomitrella patens

Characteristics: Expected / apparent Molecular Weight of the Antigene: 60 kDa
Detection limit is 12.25 pg, 7 fmol, linear range of logit/log plot is 0.02-10 pmol,midrange (B/ B o =50 %) 44.28 pg, 253 fmol, unspecific binding 2.5%.

Purification: affinity purified

Immunogen: Beta amylase isolated and purified from sweet potato Q94EU9

Isotype: IgG

Application Details

Application Notes: 1: 1000 to 1: 4000 for techniques listed above

Comment:

antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 1 mL of sterile destilled water.

Concentration: 10 mg/mL

Buffer: PBS labelled with biotin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for Amylase beta

Target: Amylase beta (amyB)

Alternative Name: Beta-amylase (amyB Products)

Background: Beta amylase (EC 3.2.1.2.) catalyzes the hydrolysis of the second alfa-1,4 glycosidic bond. Alternative names 1,4-alfa-D-glucan maltohydrolase, glycogenase,saccharogen. amylase).

Molecular Weight: 60 kDa

UniProt: Q94EU9