Expected / apparent Molecular Weight of the Antigene: 101.4 / 100 kDa (Synechocystis)
Purification
serum
Immunogen
recombinant slr0156 protein, derived from Synechocystis PCC 6803 strain slr0156 sequence. This protein is annotated as ClpB1 in a data base but was originally named ClpB2 according to the paper of Giese and Vierling (2002)
Application Notes
Recommended Dilution 1 : 3000 with standard ECL (WB).
Restrictions
For Research Use only
Format
Lyophilized
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Target
CLPB2
(ClpB2 Gene Product (CLPB2))
Alternative Name
ATP-dependent chaperone clpB2 (ClpB2)
Background
ClpB2 is essential for organism and can not be complemented by a mutation in ClpB1 gene. This cytoplasmic protein is not involved in thermotolerance. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem, 277(48): 46310-8.