No cross-reactivity with: Chlamydomonas reinhardtii
Predicted Reactivity
cyanobacteria
Characteristics
Expected / apparent Molecular Weight of the Antigene: 85.4 kDa and 95 kDa for Synechocystis
Purification
serum
Immunogen
recombinant clpB1 protein, derived from Synechocystis PCC 6803 strain slr1641 sequence, protein has an internal translation site
Application Notes
Recommended Dilution: 1 : 3000 with standard ECL (WB).
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 100 µL of sterile water
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Target
CLPB1
(ClpB1 Gene Product (CLPB1))
Alternative Name
ATP-dependent chaperone clpB1 (ClpB1)
Background
ClpB protein is essential for resistance to high temperature stress. It functions to dissolve inactive protein aggregates that accumulate at high temperatures. Giese and Vierling (2002) Changes in oligomerization are essential for the chaperone activity of a small heat shock protein in vivo and in vitro. J Biol Chem, 277(48): 46310-8.
Molecular Weight
expected: 98.1 , 85.4 and 105 kDa, apparent: 95 kDa for Synechocystis