Ferrritin 2 antibody
Fer2
Reactivity: Chlamydomonas reinhardtii
WB
Host: Rabbit
Polyclonal
unconjugated
(1 reference)-
- Target
- Ferrritin 2 (Fer2)
- Reactivity
- Chlamydomonas reinhardtii
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- Chlamydomonas reinhardtii
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 32 kDa (with transitpeptide), 16 (monomer) and aprox. 60 kDa (trimer) for Chlamydomonas reinhardtii
- Purification
- serum
- Immunogen
- KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii
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- Application Notes
- 0.7361111111
- Comment
-
a picture with a western blot application example can be found in the reference given below
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 μL of sterile water
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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-
: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." in: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).
-
: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." in: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).
-
- Target
- Ferrritin 2 (Fer2)
- Background
- Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.
- Molecular Weight
- expected: 32 (with transitpeptide) , apparent: 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii
- UniProt
- A8WCT5
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