CDJ1 antibody (N-Term)

Catalog No. ABIN334548

Product Details anti-CDJ1 Antibody

Target: CDJ1 (Chloroplast DnaJ Protein (CDJ1))

Binding Specificity: N-Term

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: higher plants

Predicted Reactivity: Ostreococcus sp.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 40 / 40 kDa

Purification: serum

Immunogen: fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli

Application Details

Application Notes: 1. 3000 with standard ECL (WB)

Comment:

western blot has been done on 10-50 µg load per well, immunoprecipitation has been done on 5-50 µl

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-CDJ1 antibody (ABIN334548)

Long, Sommer, Allen, Lu, Merchant: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." in: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).

Willmund, Dorn, Schulz-Raffelt, Schroda: "The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C." in: Plant physiology, Vol. 148, Issue 4, pp. 2070-82, (2008) (PubMed).

Target Details for CDJ1

Target: CDJ1 (Chloroplast DnaJ Protein (CDJ1))

Alternative Name: CDJ1

Synonyms: chloroplast DnaJ-like protein antibody, CDJ1 antibody

Background: CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.

Molecular Weight: expected: 40 kDa, apparent: 40 kDa

UniProt: Q66YD3