HNF1A antibody (Internal Region)

Catalog No. ABIN334504

Product Details anti-HNF1A Antibody

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF1A antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: TCF1 / HNF1

Sequence: DELPTKKGRR NRFK

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DELPTKKGRRNRFK, from the internal region of the protein sequence according to NP_000536.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: A customer reported a weak band at approx. 75 kDa on lysate of cell line Caco-2, at a concentration of 3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HepG2 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HNF1A

Target: HNF1A (HNF1 Homeobox A (HNF1A))

Alternative Name: HNF1A (HNF1A Products)

Synonyms: HNF-1A antibody, HNF1 antibody, IDDM20 antibody, LFB1 antibody, MODY3 antibody, TCF-1 antibody, TCF1 antibody, HNF-1a antibody, AI323641 antibody, HNF1-alpha antibody, HNF1[a] antibody, Hnf-1 antibody, Hnf1alpha antibody, Tcf-1 antibody, Tcf1 antibody, HNF-1-alpha antibody, HNF-1alpha antibody, LF-B1 antibody, Lfb1 antibody, HNF-1 antibody, HNF1A antibody, tcf1 antibody, FB1b antibody, HNF1b antibody, XLFB1b1 antibody, hnf1-B antibody, lfb1b antibody, mody3 antibody, tcf1-B antibody, tcfb-A antibody, HNF-1-alpha-A antibody, HNF-1A-A antibody, LFB1a1 antibody, TCF-1-A antibody, XLFB1 antibody, XLFB1b antibody, hnf1-a antibody, hnf1a antibody, hnf1a-a antibody, hnf1a-b antibody, hnf1a.L antibody, tcf1-A antibody, xx:af244934-22s antibody, HNF1 homeobox A antibody, HNF1 homeobox A L homeolog antibody, HNF1 homeobox A S homeolog antibody, HNF1 homeobox a antibody, HNF1A antibody, Hnf1a antibody, hnf1a antibody, hnf1a.L antibody, hnf1a.S antibody

Background: TCF1, transcription factor 1, hepatic LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor , HNF1, HNF1A, LFB1, MODY3 , Interferon production regulator factor (HNF1), albumin proximal factor, hepatic nuclear factor 1, maturity onset diabetes of t

Gene ID: 6927, 21405, 24817

NCBI Accession: NP_000536

Pathways: Hormone Transport, Carbohydrate Homeostasis