s100a4 antibody (C-Term)

Catalog No. ABIN334485

Product Details anti-s100a4 Antibody

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This s100a4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: S100A4 / CAPL

Sequence: CNEFFEGFPD KQP

Specificity: Both variants (NP_002952.1, NP_062427.1) represent identical protein.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CNEFFEGFPDKQP, from the C Terminus of the protein sequence according to NP_002952.1, NP_062427.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 13 kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7 kDa according to NP_002952.1). Recommended concentration: 0.05-0.2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-s100a4 antibody (ABIN334485)

Rasanen, Sriswasdi, Valiga, Tang, Zhang, Perego, Somasundaram, Li, Speicher, Klein-Szanto, Basu, Rustgi, Speicher, Herlyn: "Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3778-92, (2013) (PubMed).

Target Details for s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Alternative Name: S100A4 (S100A4 Products)

Background: S100A4, CAPL, S100 calcium binding protein A4, 18A2, 42A, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium pr

Gene ID: 6275, 20198, 24615

NCBI Accession: NP_002952, NP_062427

Pathways: S100 Proteins