PRPF31 antibody (Internal Region)

Catalog No. ABIN334465

Product Details anti-PRPF31 Antibody

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRPF31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: PRPF31

Sequence: KELGNSLDKC KNNEN

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence KELGNSLDKCKNNEN, from the internal region of the protein sequence according to NP_056444.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in nuclear lysates of cell lines A431, HeLa, HEK293 and NIH3T3 (calculated MW of 55.4 kDa according to Human NP_056444.3 and Mouse NP_081604.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at roo

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRPF31

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Alternative Name: PRPF31 (PRPF31 Products)

Synonyms: DDBDRAFT_0219465 antibody, DDBDRAFT_0304959 antibody, DDB_0219465 antibody, DDB_0304959 antibody, PRPF31 antibody, NY-BR-99 antibody, PRP31 antibody, RP11 antibody, SNRNP61 antibody, prp31 antibody, rp11 antibody, 1500019O16Rik antibody, 2810404O06Rik antibody, AW554706 antibody, zgc:66177 antibody, pre-mRNA processing factor 31 antibody, PrpF31 U4/U6*U5 snRNP-associated pre-mRNA processing factor 31, (IC) antibody, U4/U6 small nuclear ribonucleoprotein Prp31 antibody, pre-mRNA processing factor 31 S homeolog antibody, PRP31 pre-mRNA processing factor 31 homolog (yeast) antibody, PRPF31 antibody, prpf31 antibody, PrpF31 antibody, LOC100455616 antibody, Prpf31 antibody, prpf31.S antibody

Background: PRPF31, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), DKFZp566J153, NY-BR-99, PRP31, RP11 , PRP31 pre-mRNA processing factor 31 homolog (yeast), pre-mRNA processing factor 31 homolog, pre-mRNA processing factor 31 homolog (yeast)

Gene ID: 26121, 68988

NCBI Accession: NP_056444

Pathways: Ribonucleoprotein Complex Subunit Organization