PNPLA2 antibody (Internal Region)

Catalog No. ABIN334463

Product Details anti-PNPLA2 Antibody

Target: PNPLA2 (Patatin-Like phospholipase Domain Containing 2 (PNPLA2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PNPLA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: PNPLA2 / Desnutrin

Sequence: HFNSKDELIQ AN

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HFNSKDELIQAN, from the internal region of the protein sequence according to NP_065109.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Heart lysates (calculated MW of 55.3 kDa according to NP_065109.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse, PMID: 2

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: This antibody has been successfully used in IF on Human in the following papers, PMID: 33160079.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PNPLA2

Target: PNPLA2 (Patatin-Like phospholipase Domain Containing 2 (PNPLA2))

Alternative Name: PNPLA2 (PNPLA2 Products)

Synonyms: 1110001C14Rik antibody, ATGL antibody, PEDF-R antibody, TTS-2.2 antibody, TTS2 antibody, iPLA2zeta antibody, PNPLA2 antibody, pnpla2 antibody, 0610039C21Rik antibody, Atgl antibody, RGD1309044 antibody, patatin like phospholipase domain containing 2 antibody, patatin-like phospholipase domain containing 2 antibody, PNPLA2 antibody, pnpla2 antibody, Pnpla2 antibody

Background: PNPLA2, desnutrin, patatin-like phospholipase domain containing 2, 1110001C14Rik, ATGL, DKFZp667M109, FP17548, PEDF-R, TTS-2.2, TTS2, adipose triglyceride lipase, pigment epithelium-derived factor, transport-secretion protein 2.2, triglyceride hydrolase

Gene ID: 57104

NCBI Accession: NP_065109