MGLL antibody (Internal Region)

Catalog No. ABIN334436

Product Details anti-MGLL Antibody

Target: MGLL (Monoglyceride Lipase (MGLL))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MGLL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Monoglyceride Lipase

Sequence: QDLPHLVNAD GQY

Specificity: This antibody is expected to recognize both reported isoforms (NP_009214.1, NP_001003794.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QDLPHLVNADGQY, from the internal region (near N Terminus) of the protein sequence according to NP_009214.1, NP_001003794.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver, expression of MGLL is detected in hepatocytes. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in Mouse Adipose lysates and in preliminary testing of Human, Cerebellum lysate and in lysate of cell line NIH3T3 (calculated MW of 33.3 kDa according to NP_001003794.1). Recommended concentration: 0.3-1 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MGLL

Target: MGLL (Monoglyceride Lipase (MGLL))

Alternative Name: MGLL (MGLL Products)

Background: Monoglyceride lipase, HU-K5, MGLL, MGL, lysophospholipase-like

Gene ID: 11343, 23945, 29254

NCBI Accession: NP_009214, NP_001003794

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Lipid Metabolism