HTATSF1 antibody (Internal Region)

Catalog No. ABIN334420

Product Details anti-HTATSF1 Antibody

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HTATSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: HTATSF1

Sequence: QELYGDGKDG DTQTD

Cross-Reactivity: Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QELYGDGKDGDTQTD, from the internal region (near the N Terminus) of the protein sequence according to NP_055315.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 140 kDa band observed in lysates of cell line Daudi (calculated MW of 85.9 kDa according to NP_055315.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhou and Sharp, Science.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HTATSF1

Target: HTATSF1 (HIV-1 Tat Specific Factor 1 (HTATSF1))

Alternative Name: HTATSF1 (HTATSF1 Products)

Synonyms: zgc:66252 antibody, MGC98898 antibody, HTATSF1 antibody, DKFZp468B086 antibody, 1600023H17Rik antibody, 2600017A12Rik antibody, 2700077B20Rik antibody, TAT-SF1 antibody, TATSF1 antibody, dJ196E23.2 antibody, RGD1563377 antibody, HIV-1 Tat specific factor 1 antibody, HIV-1 Tat specific factor 1 S homeolog antibody, HIV Tat-specific factor 1 homolog antibody, HIV TAT specific factor 1 antibody, htatsf1 antibody, htatsf1.S antibody, HTATSF1 antibody, LOC778641 antibody, Htatsf1 antibody

Background: HTATSF1, HIV-1 Tat specific factor 1 , RP1-196E23.2, TAT-SF1, dJ196E23.2, HIV TAT specific factor 1, cofactor required for Tat activation of HIV-1 transcription

Gene ID: 27336, 317612

NCBI Accession: NP_055315