NR1H4 antibody (Internal Region)
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- Target See all NR1H4 Antibodies
- NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NR1H4 antibody is un-conjugated
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Application
- ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Farnesoid X receptor / FXR
- Sequence
- KSCREKTELT PDQQ
- Cross-Reactivity
- Cow, Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence KSCREKTELTPDQQ, from the internal region of the protein sequence according to NP_005114.1.
- Isotype
- IgG
- Top Product
- Discover our top product NR1H4 Primary Antibody
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- Application Notes
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Western Blot: Preliminary testing showed a band at approx 55 kDa in Human Kidney, Colon, Duodenum and Ileum lysate after 0.5 μg/mL antibody staining (calculated MW of 54.4 kDa according to NP_005114.1). Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- NR1H4 (Nuclear Receptor Subfamily 1, Group H, Member 4 (NR1H4))
- Alternative Name
- NR1H4 (NR1H4 Products)
- Synonyms
- zgc:110190 antibody, zgc:92742 antibody, NR1H4 antibody, BAR antibody, FXR antibody, HRR-1 antibody, HRR1 antibody, RIP14 antibody, AI957360 antibody, Fxr antibody, Rxrip14 antibody, nuclear receptor subfamily 1, group H, member 5 S homeolog antibody, nuclear receptor subfamily 1, group H, member 4 antibody, nuclear receptor subfamily 1 group H member 4 antibody, nr1h5.S antibody, nr1h4 antibody, NR1H4 antibody, Nr1h4 antibody
- Background
- Farnesoid X receptor, HRR1, NR1H4, nuclear receptor subfamily 1, group H, member 4 , BAR, FXR, HRR-1, RIP14 , farnesol receptor HRR-1
- Gene ID
- 9971
- NCBI Accession
- NP_005114
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Carbohydrate Metabolic Process
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