ALOX15 antibody (Internal Region)

Catalog No. ABIN334342

Product Details anti-ALOX15 Antibody

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ALOX15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: ALOX15

Sequence: HYKTDVAVKD DPE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HYKTDVAVKDDPE, from the internal region of the protein sequence according to NP_001131.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2 μg/mL.

Western Blot: Approx. 80 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8 kDa according to NP_001131.3). Recommended concentration: 0.2-0.5 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ALOX15

Target: ALOX15 (Arachidonate 15-Lipoxygenase (ALOX15))

Alternative Name: ALOX15 (ALOX15 Products)

Synonyms: 15-LOX-1 antibody, 15LOX-1 antibody, ALOX15 antibody, 12-LO antibody, Alox12l antibody, L-12LO antibody, 12-LOX antibody, Alox12 antibody, ALOX12 antibody, 15-LOX antibody, Alox12e antibody, arachidonate 15-lipoxygenase antibody, Arachidonate 15-lipoxygenase antibody, ALOX15 antibody, Alox15 antibody, Nmul_A0532 antibody, Sden_1680 antibody, Swoo_2919 antibody, PCC8801_3106 antibody, Cyan8802_3014 antibody, Nwat_1315 antibody

Background: ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2

Gene ID: 246

NCBI Accession: NP_001131

Pathways: Regulation of Actin Filament Polymerization