PDHA1 antibody (N-Term)

Catalog No. ABIN321377

Product Details anti-PDHA1 Antibody

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHA1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human PDHA1 / PDHA

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Guinea pig (100%) Bovine (92%).

Purification: Protein A purified

Immunogen: Synthetic peptide from N-Terminus of human PDHA1 (P08559, NP_000275). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Rabbit, Guinea pig (100%), Hamster, Bovine, Horse, Pig, Platypus (92%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 5.0 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PDHA1

Target: PDHA1 (Pyruvate Dehydrogenase (Lipoamide) alpha 1 (PDHA1))

Alternative Name: PDHA1 / PDH E1 alpha (PDHA1 Products)

Background: Name/Gene ID: PDHA1

Synonyms: PDHA1, PDHCE1A, PDHA, PHE1A, PDHE1-A type I

Gene ID: 5160

NCBI Accession: NP_000275

UniProt: P08559

Pathways: Warburg Effect