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GFP antibody

Name: GFP antibody
Brand: antibodies-online
SKU: ABIN3210020
Price: 961.13 USD
Availability: InStock

GFP Reactivity: Aequorea victoria WB, ELISA, IHC, FACS Host: Mouse Monoclonal 4B10B2 unconjugated

Western Blot showing using GFP antibody used against extracts from HCC827 cells, untransfected (1) and transfected with GFP (2). (GFP antibody)

FC analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP antibody . (GFP antibody)

FACS

Immunocytochemistry analysis of HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP antibody . (GFP antibody)

IHC

Western Blot showing GFP antibody used against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4). (GFP antibody)

4 images

Catalog No. ABIN3210020

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
176

15

11

9
Aequorea victoria
Host
64

62

35

15

9

5

1

1
Mouse
Compatible Secondaries
Clonality
115

72
Monoclonal
Conjugate
82

13

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
Application
150

76

46

33

25

25

25

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification

Purified

Immunogen

GFP antibody was raised in Mouse using a purified recombinant fragment of GFP expressed in E. coli as the immunogen.

Clone

4B10B2

Isotype

IgG2a

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Application Notes

ELISA: 1:10,000, FC: 1:200-1:400, IHC: 1:200-1:1000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

Purified antibody supplied in PBS containing 0.03 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C for short term storage. Store at -20 °C for long term storage.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Green Fluorescent Protein (GFP) tagging provide an excellent means for monitoring gene expression and protein localization in living cells, so that it is widely accepted by molecular and cell biological research. Monoclonal anti-GFP antibody can detect GFP and its variants on Western blotting, Immunoprecipitation and Immunocytochemistry. Synonyms: Monoclonal GFP antibody, Anti-GFP antibody, Green Fluorescent Protein antibody.