PUF60 antibody (AA 371-420)

Catalog No. ABIN320588

Product Details anti-PUF60 Antibody

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Binding Specificity: AA 371-420

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUF60 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Human PUF60

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Horse, Guinea pig (100%).

Purification: Protein A purified

Immunogen: Synthetic peptide located between aa371-420 of human PUF60 (Q9UHX1-2, NP_055096). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Opossum, Guinea pig (100%), Orangutan, Bat (92%), Turkey, Zebra finch, Chicken, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Alternative Name: PUF60 (PUF60 Products)

Synonyms: FIR antibody, RoBPI antibody, SIAHBP1 antibody, fc21a05 antibody, wu:fc21a05 antibody, wu:fi43a08 antibody, 2410104I19Rik antibody, 2810454F19Rik antibody, Siahbp1 antibody, fe37c05 antibody, repressor antibody, si:ch211-12p8.2 antibody, si:zc12p8.2 antibody, wu:fb33e11 antibody, wu:fe37c05 antibody, zgc:86806 antibody, poly(U) binding splicing factor 60 antibody, poly-U binding splicing factor a antibody, poly-U binding splicing factor 60 antibody, poly-U binding splicing factor b antibody, PUF60 antibody, puf60a antibody, Puf60 antibody, puf60b antibody

Background: Name/Gene ID: PUF60

Synonyms: PUF60, FBP-interacting repressor, FBP interacting repressor, RoBPI, Siah-BP1, Ro-binding protein 1, RoBP1, Siah binding protein 1, FIR, Siah-binding protein 1, SIAHBP1

Gene ID: 22827

NCBI Accession: NP_055096

UniProt: Q9UHX1