CD3D antibody (AA 22-106)
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- Target See all CD3D Antibodies
- CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))
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Binding Specificity
- AA 22-106
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD3D antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to Cluster Of Differentiation 3d (CD3d)
- Sequence
- MGHHHHHHSG SEF-FKIQVTEYE DKVFVTCNTS VMHLDGTVEG WFAKNKTLNL GKGVLDPRGI YLCNGTEQLA KVVSSVQVHY RMCQNCVELD SGTM
- Specificity
- The antibody is a mouse monoclonal antibody raised against CD3d. It has been selected for its ability to recognize CD3d in immunohistochemical staining and western blotting.
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Cluster Of Differentiation 3d (CD3d) corresdonding to Phe22~Gly106 ((Accession # P04235)
- Clone
- 2#
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product CD3D Primary Antibody
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- Application Notes
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Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))
- Alternative Name
- CD3d (CD3D Products)
- Synonyms
- CD3-DELTA antibody, T3D antibody, CD3 antibody, T3d antibody, CD3d molecule antibody, CD3 antigen, delta polypeptide antibody, CD3D antibody, Cd3d antibody
- Background
- CD3-DELTA, T3D, T-Dell Surface Glycoprotein CD3 Delta Chain, T-cell receptor T3 delta chain
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events
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