S100B antibody (AA 1-92)
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- Target See all S100B Antibodies
- S100B (S100 Calcium Binding Protein B (S100B))
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Binding Specificity
- AA 1-92
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This S100B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to S100 Calcium Binding Protein B (S100B)
- Sequence
- MGHHHHHHSG SEF-MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE QEVVDKVMET LDEDGDGECD FQEFMAFVSM VTTACHEFFE HE
- Specificity
- The antibody is a mouse monoclonal antibody raised against S100B. It has been selected for its ability to recognize S100B in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Mouse
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant S100 Calcium Binding Protein B (S100B) corresdonding to Met1~Glu92 with N-terminal His Tag
- Clone
- D5
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product S100B Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- S100B (S100 Calcium Binding Protein B (S100B))
- Alternative Name
- S100 Calcium Binding Protein B (S100B Products)
- Synonyms
- NEF antibody, S100 antibody, S100-B antibody, S100beta antibody, S100P antibody, AI850290 antibody, Bpb antibody, si:dkey-110p14.2 antibody, wu:fq18b10 antibody, zgc:92776 antibody, S100 calcium binding protein B antibody, S100 protein, beta polypeptide, neural antibody, S100 calcium binding protein, beta (neural) antibody, S100B antibody, S100b antibody, s100b antibody
- Background
- S100-B, NEF, S100Beta, S-100 protein subunit beta
- Pathways
- Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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