TNF alpha antibody (AA 80-235)
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- Target See all TNF alpha Antibodies
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Binding Specificity
- AA 80-235
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TNF alpha antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- L RSSSQNSSDK PVAHVVANHQ AEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLIYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVS LLSAIKSPCP KDTPEGAELK PWYEPMYLGG VFQLEKGDLL SAEVNLPKYL DITESGQVYF GVIAL
- Specificity
- The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse
- Purification
- Protein A + Protein G affinity chromatography
- Immunogen
- Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Leu80~Leu235 with N-terminal His Tag
- Clone
- D3
- Isotype
- IgG2b kappa
- Top Product
- Discover our top product TNF alpha Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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Inflammatory response and neuronal necrosis in rats with cerebral ischemia." in: Neural regeneration research, Vol. 9, Issue 19, pp. 1753-62, (2014) (PubMed).
: "
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Inflammatory response and neuronal necrosis in rats with cerebral ischemia." in: Neural regeneration research, Vol. 9, Issue 19, pp. 1753-62, (2014) (PubMed).
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- Target
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- Alternative Name
- Tumor Necrosis Factor Alpha (TNF alpha Products)
- Synonyms
- DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody
- Background
- DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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