Recognises Murine Beta-Glucan Receptor (Dectin-1). This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version SM2025LE is recommended for Functional Studies.
Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line2
CLEC7A
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
Flow Cytometry (Neat -1/10): Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1 mL distilled water
Buffer
PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA and 5 % sucrose as stabilizers.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Target
CLEC7A
(C-Type Lectin Domain Family 7, Member A (CLEC7A))
Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.Synonyms: BGR, Beta-glucan receptor, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell-associated C-type lectin 1