EID1 antibody (AA 159-187)

Catalog No. ABIN3201016

Product Details anti-EID1 Antibody

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Binding Specificity: AA 159-187

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EID1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity: Reacts with human, mouse and rat EID1 proteins

Purification: This antibody was purified by our propriety chromatography under mild conditions as IgG fraction from serum-free growth medium of hybridoma clone #26.

Sterility: Sterile filtered

Immunogen: Synthetic peptide containing aa 159-187 of human DIE1 protein

Clone: 26

Isotype: IgG2a kappa

Application Details

Application Notes: 1) Western blotting: ~1 g/mL
2) Immunofluorescence staining: 1~5 g/mL
3) ELISA
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol. Azide- and carrier-free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-EID1 antibody (ABIN3201016)

Nguyen, Baglia, Huang, Baker, McCance: "Acetylation regulates the differentiation-specific functions of the retinoblastoma protein." in: The EMBO journal, Vol. 23, Issue 7, pp. 1609-18, (2004) (PubMed).

MacLellan, Xiao, Abdellatif, Schneider: "A novel Rb- and p300-binding protein inhibits transactivation by MyoD." in: Molecular and cellular biology, Vol. 20, Issue 23, pp. 8903-15, (2000) (PubMed).

Target Details for EID1

Target: EID1 (EP300 Interacting Inhibitor of Differentiation 1 (EID1))

Alternative Name: EID1 (EID1 Products)

Synonyms: C15orf3 antibody, CRI1 antibody, EID-1 antibody, IRO45620 antibody, PTD014 antibody, RBP21 antibody, 2610002K20Rik antibody, Cri1 antibody, ORF12 antibody, PNAS-22 antibody, RGD1562702 antibody, EP300 interacting inhibitor of differentiation 1 antibody, EID1 antibody, Eid1 antibody

Background: EID1 (EP300 interacting inhibitor of differentiation 1) encodes a 21 kDa protein, which interacts with RB1 and EP300 and acts as a repressor of MYOD1 transcription. EID1 inhibits EP300 and CBP histone acetyltransferase activity. It may be involved in coupling cell cycle exit to the transcription of genes required for cellular differentiation.

UniProt: Q9Y6B2