p53 antibody (pSer315)

Catalog No. ABIN3201006

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: pSer315

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: This product is the purified IgG fraction obtained from serum-free culture medium of mouse hybridoma (clone #18) which produces monoclonal antibody that specifically recognizes human p53 protein with phospholyrated Ser315.

Purification: Purified

Immunogen: Synthetic peptide containing pSer315 of p53

Clone: 18

Isotype: IgG2b kappa

Application Details

Application Notes: 1. Western blotting: 1,000~2000 dilution

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS ( pH 7.4), 50 % glycerol

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-p53 antibody (ABIN3201006)

Bode, Dong: "Post-translational modification of p53 in tumorigenesis." in: Nature reviews. Cancer, Vol. 4, Issue 10, pp. 793-805, (2004) (PubMed).

Katayama, Sasai, Kawai, Yuan, Bondaruk, Suzuki, Fujii, Arlinghaus, Czerniak, Sen: "Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and inhibition of p53." in: Nature genetics, Vol. 36, Issue 1, pp. 55-62, (2003) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53 Products)

Background: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Ser315 is phosphorylated by aurora kinase and cycline-dependent kinases when cells are subjected to stress such as DNA damage and microtubule disruption by nocodazole. However the effect of the phosphorylation on the function of p53 is mostly unknown.

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect