p53 antibody (pSer46)

Catalog No. ABIN3201005

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: pSer46

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity (Details): Not tested in other species.

Purification: Purified from serum-free culture medium of hybridoma (#36) by proprietary chromatography procedures under mild conditions.

Sterility: Sterile filtered

Immunogen: Synthetic peptide containing pSer46 of p53

Clone: 36

Isotype: IgG1 kappa

Application Details

Application Notes: 1) Western blotting: 1,000~2,000 dilution
2) Immunohistochemistry (assay dependent)
3) ELISA
Other applications have not been tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (x1), 50 % glycerol. Azide and carrier free.

Preservative: Azide free

Storage: -20 °C

Storage Comment: Upon arrival centrifuge briefly and store at -20 C.

References for anti-p53 antibody (ABIN3201005)

Taira, Yamamoto, Yamaguchi, Miki, Yoshida: "ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4909-19, (2010) (PubMed).

Kodama, Otsubo, Hirota, Yokota, Enari, Taya: "Requirement of ATM for rapid p53 phosphorylation at Ser46 without Ser/Thr-Gln sequences." in: Molecular and cellular biology, Vol. 30, Issue 7, pp. 1620-33, (2010) (PubMed).

Taira, Nihira, Yamaguchi, Miki, Yoshida: "DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage." in: Molecular cell, Vol. 25, Issue 5, pp. 725-38, (2007) (PubMed).

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53 Products)

Synonyms: BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody

Background: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI As to the kinase of phosphorylation of Ser46, involvement of DYRK2 and ATM have been implicated.

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect