COXIIB antibody

Catalog No. ABIN3197486

Product Details anti-COXIIB Antibody

Target: COXIIB (Cytochrome Oxidase Subunit II B (COXIIB))

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Characteristics: Expected / apparent Molecular Weight of the Antigene: 15 kDa

Purification: serum

Immunogen: GST fusion with the aminoacids 4-153 of the subunit 2b of the Chlamydomonas reinhardtii cytochrome oxidase, UniProt: Q9AU05

Application Details

Application Notes: 1: 5000 with alkaline phosphatase (WB), 1: 25 000 with standard ECL (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-COXIIB antibody (ABIN3197486)

Johnson, Steinbeck, Dent, Takahashi, Richaud, Ozawa, Houille-Vernes, Petroutsos, Rappaport, Grossman, Niyogi, Hippler, Alric: "Proton gradient regulation 5-mediated cyclic electron flow under ATP- or redox-limited conditions: a study of ΔATpase pgr5 and ΔrbcL pgr5 mutants in the green alga Chlamydomonas reinhardtii." in: Plant physiology, Vol. 165, Issue 1, pp. 438-52, (2014) (PubMed).

Terauchi, Peers, Kobayashi, Niyogi, Merchant: "Trophic status of Chlamydomonas reinhardtii influences the impact of iron deficiency on photosynthesis." in: Photosynthesis research, Vol. 105, Issue 1, pp. 39-49, (2010) (PubMed).

Diaz-Troya, Florencio, Crespo: "Target of rapamycin and LST8 proteins associate with membranes from the endoplasmic reticulum in the unicellular green alga Chlamydomonas reinhardtii." in: Eukaryotic cell, Vol. 7, Issue 2, pp. 212-22, (2008) (PubMed).

Target Details for COXIIB

Target: COXIIB (Cytochrome Oxidase Subunit II B (COXIIB))

Background: COXII - mitochondrial cytochrome oxidase subunit II is located in inner mitochondrial membrane. The enzyme is a component of the respiratory chain complex involved in the transfer of electrons from cytochrome c to oxygen.

Molecular Weight: 15 kDa