No cross-reactivity with: Chlamydomonas reinhardtii
Characteristics
Expected / apparent Molecular Weight of the Antigene: 36-40 / 36-40 for Arabidopsis thaliana
Purification
serum
Immunogen
KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms 1 and 2 including Arabidopsis thaliana AOX1A (At3g22370) and AOX2 (At3g64210), Solanum lycopersicum Q7XBG9, Oryza sativa Q7XT33,
Application Notes
Recommended Dilution: 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection(WB).
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 200 µL of sterile water
Handling Advice
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage
-20 °C
Barreto, Okura, Neshich, Maia, Arruda: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
Tsaniklidis, Delis, Nikoloudakis, Katinakis, Aivalakis: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).
Zubo, Potapova, Yamburenko, Tarasenko, Konstantinov, Börner: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." in: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).
Wang, Vanlerberghe: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).
Kornfeld, Heskel, Atkin, Gough, Griffin, Horton, Turnbull: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." in: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).
Zhang, Peck: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
Wang, Li, Liu, He, Bi: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).
Target
AOX1/AOX2
(Alternative Oxidase 1/2 (AOX1/AOX2))
Alternative Name
Plant alternative oxidase 1 and 2 (AOX1/2)
Synonyms
aox2 antibody, ALTERNATIVE OXIDASE antibody, MSJ1.5 antibody, MSJ1_5 antibody, alternative oxidase 2 antibody, F27G20.12 antibody, alternative oxidase 1D antibody, Afu2g05060 antibody, alternative oxidase 1 antibody, ubiquinol oxidase 1, mitochondrial antibody, alternative oxidase 2 antibody, alternative oxidase 1D antibody, alternative oxidase AlxA antibody, alternative oxidase antibody, aox1 antibody, AOX1 antibody, AOX2 antibody, AOX1D antibody, CNA01500 antibody, aox antibody, AFUA_2G05060 antibody, Mmar10_2564 antibody, PTOX1 antibody, Cseg_3042 antibody, VOLCADRAFT_65634 antibody
Background
AGI Code: At3g22370 Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water.
Molecular Weight
expected: 36-40 kDa, apparent: 36-40 for Arabidopsis thaliana