This Macrophage Scavenger Receptor 1 antibody is conjugated to FITC
Application
Flow Cytometry (FACS)
Specificity
This antibody recognises the Murine Scavenger Receptor class A (SRA), type I and II, also known as CD204. Clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).
Purification
Affinity Chromatography on Protein G.
Immunogen
RAW264 cell line. Remarks: Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line.
MSR1
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion. Recent research shows that clone 2F8 recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.Synonyms: MSR1, Macrophage acetylated LDL receptor I and II, Macrophage scavenger receptor types I and II, SCARA1, Scavenger receptor class A member 1