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AKT1 antibody (pThr450)
AKT1
Reactivity: Human, Mouse, Rat
IF, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-AKT1 Antibody
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Target
See all AKT1 Antibodies
AKT1
(V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
Binding Specificity
All epitopes for AKT1 antibodies
pThr450
Reactivity
All reactivities for AKT1 antibodies
Human, Mouse, Rat
Host
All hosts for AKT1 antibodies
Rabbit
Clonality
All clonalities for AKT1 antibodies
Polyclonal
Conjugate
All conjugates for AKT1 antibodies
This AKT1 antibody is un-conjugated
Application
All applications for AKT1 antibodies
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
AKT1 (phospho-Thr450) Antibody AP08031PU detects endogenous levels of AKT1 only when phosphorylated at Threonine 450.
Purification
Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).
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Discover our top product AKT1 Primary Antibody
Alternatives
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Application Details
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Application Notes
Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Handling
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Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the antibody (in aliquots) at-20 °C.
Target Details for AKT1
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Target
AKT1
(V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))
Alternative Name
AKT1 / PKB (AKT1 Products )
Synonyms
AKT antibody, CWS6 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, Akt antibody, Rac antibody, PKB/Akt antibody, PKBalpha antibody, AKT/PKB antibody, AKT1 antibody, Akt/PKB antibody, Akt1 antibody, CG4006 antibody, D-Akt antibody, DAKT1 antibody, DAKT1/PKB antibody, DAkt antibody, DAkt1 antibody, DPKB antibody, DRAC-PK antibody, DRAC-PK66 antibody, DRAC-PK85 antibody, Dakt antibody, Dakt1 antibody, Dmel\\CG4006 antibody, Dpkb antibody, PKB/AKT antibody, PKB/dAKT antibody, RacPK antibody, akt antibody, akt1 antibody, dAKT antibody, dAKT/dPKB antibody, dAKT1 antibody, dAkt antibody, dAkt/PKB antibody, dAkt1 antibody, dPKB antibody, dakt antibody, dakt1 antibody, l(3)04226 antibody, l(3)89Bq antibody, p-Akt antibody, pAkt antibody, ACT-5 antibody, akt-1 antibody, pkb antibody, v-akt antibody, v-akt1 antibody, xAct antibody, ATAKT1 antibody, F18A8.2 antibody, F18A8_2 antibody, K+ transporter 1 antibody, POTASSIUM TRANSPORTER antibody, AKT serine/threonine kinase 1 antibody, thymoma viral proto-oncogene 1 antibody, CG4006 gene product from transcript CG4006-RE antibody, actin beta antibody, v-akt murine thymoma viral oncogene homolog 1 S homeolog antibody, v-akt murine thymoma viral oncogene homolog 1 antibody, K+ transporter 1 antibody, Serine/threonine-protein kinase akt-1 antibody, AKT1 antibody, Akt1 antibody, ACTB antibody, akt1.S antibody, akt1 antibody, KT1 antibody, akt-1 antibody
Background
Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID
207
NCBI Accession
NP_001014431
UniProt
P31749
Pathways
PI3K-Akt Signaling , RTK Signaling , TCR Signaling , AMPK Signaling , Interferon-gamma Pathway , TLR Signaling , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Response to Water Deprivation , Regulation of Actin Filament Polymerization , Carbohydrate Homeostasis , Glycosaminoglycan Metabolic Process , Cellular Glucan Metabolic Process , Regulation of Muscle Cell Differentiation , Cell-Cell Junction Organization , Regulation of Cell Size , Skeletal Muscle Fiber Development , Regulation of Carbohydrate Metabolic Process , Hepatitis C , Protein targeting to Nucleus , CXCR4-mediated Signaling Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Negative Regulation of intrinsic apoptotic Signaling , Thromboxane A2 Receptor Signaling , Signaling of Hepatocyte Growth Factor Receptor , Positive Regulation of fat Cell Differentiation , VEGFR1 Specific Signals , VEGF Signaling , Warburg Effect
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