This antibody is specific for p38 mitogen-activated protein kinase (p38 MAPK), also known as MAPK14, when phosphorylated at Threonine 180 and Tyrosine 182.
Cross-Reactivity (Details)
Species reactivity (expected):Chicken, Zebrafish, Bovine, Monkey, Mouse and Canine (Dog). Species reactivity (tested):Human and Rat.
Purification
Affinity Chromatography.
Immunogen
Synthetic phosphopeptide corresponding to an amino acid sequence within p38 MAPK which includes phosphorylated Thr180 and Tyr182.
MAPK14
Reactivity: Human
WB, IHC, IF, IP
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1/1000. This p38 MAPK antibody Recognises a band of approximately 39 kD in Western blots ofanisomycin C-6 glioma cell lysates. For the detection of phosphoproteins, Threonine and Tyrosine phosphatase inhibitors suchas 10 mM Sodium Fluoride and 1 mM Sodium Orthovanadate should be added to the samplebuffer. Milk or other casein-based blocking solutions are not recommended as casein is aphosphoprotein and its use can result in high background. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % BSA and 50 % Glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
MAPK14
(Mitogen-Activated Protein Kinase 14 (MAPK14))
P38 MAPK is a serine/threonine kinase which plays an important role in signal transduction, contributing to the regulation of many cellular processes including cell differentiation and inflammation. The protein is activated by phosphorylation of threonine 180 and tyrosine 182, by several upstream kinases, in response to a wide range of extracellular stimuli such as UV B irradiation or endotoxin exposure.Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha