CD44 antibody

Catalog No. ABIN318928

Product Details anti-CD44 Antibody

Target: CD44

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody specifically recognises epitopes encoded by exons v3-v10 on the variant portion of CD44.

Purification: Purified

Immunogen: Recombinant human CD44v3-v10

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on frozen sections: 50g/mL. Immunohistochemistry on paraffin sections: 20g/mL, requires antigen retrieval using heattreatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 isrecommended for this purpose. Positive Control: Breast carcinoma and colon adenocarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD44

Target: CD44

Alternative Name: CD44 (CD44 Products)

Synonyms: CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody

Background: CD44 is a ubiquitously expressed type 1 transmembrane glycoprotein and proteoglycan, otherwise known as phagocytic glycoprotein 1 (pgp 1), which acts as a receptor for the major extracellular matrix component hyaluronate, and also binds other ligands including collagen and matrix metalloproteinases (MMPs). CD44 is known to play a role in cell-cell interactions, cell adhesion, the activation and homing of lymphocytes, cell migration, haematopoiesis and metastasis, and exists as a number of isoforms generated by the alternative RNA splicing of at least 10 variant exons (CD44v).Synonyms: CD44, CDw44, ECMR-III, Epican, Extracellular matrix receptor-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, LHR, MDU2, MDU3, MIC4, PGP-1, Phagocytic glycoprotein I

Gene ID: 960

NCBI Accession: NP_000601

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling