Complement C4A/C4B (AA 1241-1256) antibody

Catalog No. ABIN318426

Product Details

Target: Complement C4A/C4B

Binding Specificity: AA 1241-1256

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Specificity: This antibody is specific for complement split product C4d.

Purification: Purified Ig prepared by affinity chromatography on Protein G

Immunogen: Synthetic peptide corresponding to amino acids 1241-1256 of C4 conjugated to Keyhole limpet Haemocyanin.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1/20 - 1/80. Immunohistochemistry on paraffin sections: 1/20 - 1/80, Heat induced epitope retrieval isrecommended. Immunofluorescence: 1/20 - 1/80.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Complement C4A/C4B

Synonyms: C4 antibody, C4-1 antibody, C4b antibody, complement component 4A (Rodgers blood group) antibody, C4a antibody

Background: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.Synonyms: CPAMD2, CPAMD3, Complement Component 4

Gene ID: 720

NCBI Accession: NP_009224

UniProt: P0C0L4