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FITC antibody (Biotin)

FITC IHC (fro) Host: Sheep Polyclonal Biotin
Catalog No. ABIN318342
  • Target See all FITC products
    FITC (Fluorescein Isothiocyanate (FITC))
    Reactivity
    Please inquire
    Host
    • 40
    • 17
    • 16
    • 10
    • 8
    Sheep
    Clonality
    • 49
    • 41
    Polyclonal
    Conjugate
    • 28
    • 14
    • 13
    • 13
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FITC antibody is conjugated to Biotin
    Application
    • 55
    • 38
    • 31
    • 17
    • 15
    • 14
    • 14
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (Frozen Sections) (IHC (fro))
    Purification
    Purified IgG prepared by affinity chromatography.
    Immunogen
    Fluorescein isothiocyanate (isomer 1)
    Isotype
    IgG
  • Application Notes
    Immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    PBS, 0.09 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    FITC (Fluorescein Isothiocyanate (FITC))
    Alternative Name
    FITC (FITC Products)
    Target Type
    Chemical
    Background
    FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.Synonyms: Fluorescein Isothiocyanate
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