p53 antibody (pSer9)

Catalog No. ABIN318096

Product Details anti-p53 Antibody

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: pSer9

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of p53 when phosphorylated at Ser9.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53 (TP53) (TP53 Products)

Background: The p53 protein was initially identified in a complex with the SV40 large T antigen in SV40-transformed rodent cells. Subsequently, p53 was shown to form complexes with adeno-virus E1B protein and the E6 protein encoded by oncogenic papillomavirus variants. Although p53 was initially considered to be a cellular proto-oncogene, it has since been classified as a tumor suppressor gene or anti-oncogene. Presumably, the presence of excess amounts of the mutant form of the protein can lead to functional inactivation of wild type p53. Mutations and allelic loss of the p53 gene have been associated with malignant transformation in a wide variety of human tumors. These findings in combination with the fact that p53 can act as a negative regulator of cell growth in normal cells suggest that the binding of p53 by various DNA tumor virus-encoded transforming proteins may be a major contributing factor to their transforming phenotype.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Molecular Weight: approx. 48 kDa

Gene ID: 7157

NCBI Accession: NP_000537

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect