REM1 antibody

Catalog No. ABIN317842

Product Details anti-REM1 Antibody

Target: REM1 (RAS (RAD and GEM)-Like GTP-Binding 1 (REM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of Rad GTPase protein.(region surrounding Leu80)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for REM1

Target: REM1 (RAS (RAD and GEM)-Like GTP-Binding 1 (REM1))

Alternative Name: REM1 (REM1 Products)

Synonyms: GD:REM antibody, GES antibody, E030011C07Rik antibody, Rem antibody, GEM antibody, SI:zK76P14.3 antibody, horizin antibody, rem antibody, zgc:56144 antibody, RRAD and GEM like GTPase 1 antibody, rad and gem related GTP binding protein 1 antibody, RAS (RAD and GEM)-like GTP-binding 1 antibody, REM1 antibody, Rem1 antibody, rem1 antibody

Background: The Ras-encoded family of proteins bind GDP and GTP with high affinity. They possess a low level of intrinsic GTPase activity that increases more than 100-fold when interacting with cytosolic GTPase activating protein (GAP). Ras family members include H-Ras, K-Ras, N-Ras, M-Ras, R-Ras, ERas, Rheb, TC 21, RASL11B and Rad (Ras associated with diabetes) GTPase.Rad GTPase is a GTP-binding protein that is similar to Ras but has unique features. Unlike other small GTPases, Rad GTPase lacks typical pren-ylation motifs at its C terminus. The Rad GTPase enzyme binds calmodulin, inhibits vascular lesion formation, has low intrinsic GTPase activity and cannot be stimulated by any known GAP molecules. Rad GTPase is expressed in skeletal muscle, cardiac muscle and lung tissues and is overexpressed in the skeletal muscle tissue of individuals with type II diabetes.Synonyms: GES, GTP-binding protein REM 1, GTPase-regulating endothelial cell sprouting, REM, Rad and Gem-like GTP-binding protein 1

Molecular Weight: approx. 33 kDa

Gene ID: 28954

NCBI Accession: NP_054731

UniProt: O75628