S1PR2 antibody

Catalog No. ABIN317746

Product Details anti-S1PR2 Antibody

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of EDG-5 protein.(region surrounding Leu300)

Cross-Reactivity (Details): Species reactivity (expected):Rat and Mouse.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/40000approx. 1/60000. Western Blot: 1/500-1/1000. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-S1PR2 antibody (ABIN317746)

Pérez-Cerezales, Boryshpolets, Afanzar, Brandis, Nevo, Kiss, Eisenbach: "Involvement of opsins in mammalian sperm thermotaxis." in: Scientific reports, Vol. 5, pp. 16146, (2016) (PubMed).

Duarte, Kobayashi, Kawamoto, Moriyama: "RELAXIN enhances differentiation and matrix mineralization through Relaxin/insulin-like family peptide receptor 2 (Rxfp2) in MC3T3-E1 cells in vitro." in: Bone, Vol. 65, pp. 92-101, (2015) (PubMed).

Yao, Peng, Dai: "The role of hepatocyte nuclear factor 4alpha in metastatic tumor formation of hepatocellular carcinoma and its close relationship with the mesenchymal-epithelial transition markers." in: BMC cancer, Vol. 13, pp. 432, (2014) (PubMed).

Wang, Li, Pan, Zhu, Wang: "Influence of hypercapnia on the synthesis of neuropeptides and their receptors in murine brain." in: Respirology (Carlton, Vic.), Vol. 18, Issue 1, pp. 102-7, (2013) (PubMed).

Huang, Liu, Lan, Xie, Peng, Huang, Wang, Shen, Liu, Huang: "Berberine reduces fibronectin expression by suppressing the S1P-S1P2 receptor pathway in experimental diabetic nephropathy models." in: PLoS ONE, Vol. 7, Issue 8, pp. e43874, (2012) (PubMed).

Niwa, Rowbotham, Lambert: "Evaluation of primary opioid receptor antibodies for use in western blotting." in: British journal of anaesthesia, Vol. 108, Issue 3, pp. 530-2, (2012) (PubMed).

Target Details for S1PR2

Target: S1PR2 (Sphingosine-1-Phosphate Receptor 2 (S1PR2))

Alternative Name: EDG-5 / S1P2 (S1PR2 Products)

Synonyms: AGR16 antibody, EDG-5 antibody, EDG5 antibody, Gpcr13 antibody, H218 antibody, LPB2 antibody, S1P2 antibody, 1100001A16Rik antibody, Edg5 antibody, LPb2 antibody, GPCR18 antibody, snGPCR18 antibody, sphingosine-1-phosphate receptor 2 antibody, S1PR2 antibody, S1pr2 antibody

Background: The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.Synonyms: AGR16, EDG5, Endothelial differentiation G-protein coupled receptor 5, Gpcr13, H218, LPB2, S1P receptor 2, S1PR2, Sphingosine 1-phosphate receptor 2

Molecular Weight: approx. 39 kDa

Gene ID: 9294

NCBI Accession: NP_004221

UniProt: O95136

Pathways: Synaptic Membrane